Parallel antiport mechanisms for Na+ and Cl- transport in herbivorous teleost intestine

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Parallel antiport mechanisms for Na+ and Cl- transport in herbivorous teleost intestine.

Transport mechanisms for NaCl in intestinal brush border membrane vesicles (BBMV) of herbivorous, seawater-adapted tilapia, Oreochromis mossambicus, were investigated with a rapid filtration technique using 22Na+ and 36Cl- as tracers. Evidence to support the existence of cotransport mechanisms (Na+/Cl- or Na+/K+/2Cl-) in tilapia intestinal BBMV was not found. An inwardly directed Cl- gradient o...

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Short-chain fatty acid (SCFA) concentrations of intestinal contents from the herbivorous tilapia, Oreochromis mossambicus, were analysed by gas and high-performance liquid chromatography. Concentrations of acetate ranging from 3 to 18 mmol l-1 were found along the full length of the intestinal tract, as were trace amounts of propionate. Characteristics of [3H]acetate transport by the intestinal...

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Basolateral membrane Na+/H+ antiport, Na+/base cotransport, and Na+-independent Cl-/base exchange in the rabbit S3 proximal tubule.

The basolateral membrane Na+ and Cl(-)-dependent acid-base transport processes were studied in the isolated perfused rabbit S3 proximal straight tubule. Intracellular pH (pHi) was measured with 2'7'-biscarboxyethyl-5,6-carboxyfluorescein (BCECF) and a microfluorometer coupled to the tubule perfusion apparatus. Reduction of basolateral HCO3- from 25 to 5 mM caused pHi to decrease at a rate of 0....

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Basolateral membrane Cl(-)-, Na(+)-, and K(+)-coupled base transport mechanisms in rat MTALH.

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Evidence for an apical Na-Cl cotransporter involved in ion uptake in a teleost fish.

Cation-chloride cotransporters, such as the Na(+)/K(+)/2Cl(-) cotransporter (NKCC) and Na(+)/Cl(-) cotransporter (NCC), are localized to the apical or basolateral plasma membranes of epithelial cells and are involved in active ion absorption or secretion. The objectives of this study were to clone and identify ;freshwater-type' and ;seawater-type' cation-chloride cotransporters of euryhaline Mo...

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ژورنال

عنوان ژورنال: Journal of Experimental Biology

سال: 1988

ISSN: 1477-9145,0022-0949

DOI: 10.1242/jeb.135.1.65